Part:BBa_K4147005

Dermaseptin B1 (AKA MsrA2) expression construct with LacI regulated promoter

This part contains the CDS of the antimicrobial peptide Dermaseptin B1 (BBa_K2577001) under the promotion of a LacI regulated promoter (BBa_R0010). This construct also contains an RBS under the part name BBa_B0032 as well as a 6xHis tag on the C- terminal for purification with IMAC and lastly a double terminator (BBa_B0015).

Usage and Biology

This synthetic derivative of dermaseptin B1, MsrA2 (N-Met-dermaseptin B1) has proven to possess activity against phytopathogenic fungi such as Alternaria, Cercospora , Fusarium, Phytophthora, Pythium, Rhizoctonia and Verticillium sp [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Osusky, M., Osuska, L., Kay, W., & Misra, S. (2005). Genetic modification of potato against microbial diseases: in vitro and in planta activity of a dermaseptin B1 derivative, MsrA2. Theoretical and Applied Genetics, 111(4), 711–722. doi:10.1007/s00122-005-2056-y